Alpha Tubulin Molecular Weight Cst

Membrane blocking and.
Alpha tubulin molecular weight cst. Monoclonal antibody for studying tubulin alpha in the cytoskeletal signaling research area. α subunit of tubulin has molecular weight of 50 000. Immunoprecipitation of acetyl α tubulin lys40 from hela extracts treated with trichostatin a tsa 9950 1μm 18 hours. Lane 1 is 10 input lane 2 is acetyl α tubulin lys40 d20g3 xp rabbit mab and lane 3 is rabbit da1e mab igg xp isotype control 3900.
Loading of prestained molecular weight markers 13953 5 µl lane to verify electrotransfer and biotinylated protein ladder 7727 10 µl lane to determine molecular weights are recommended. Use a concentration of 1 µg ml. Western blot analysis was performed with acetyl α tubulin lys40 6 11 b 1 mouse mab 12152. Loading of prestained molecular weight markers 13953 5 µl lane to verify electrotransfer and biotinylated protein ladder 7727 10 µl lane to determine molecular weights are recommended.
Monoclonal antibody for studying tubulin alpha in the cytoskeletal signaling research area. Western blot analysis of recombinant alpha tubulin and beta tubulin gst fusion proteins and extracts from nih 3t3 and c6 cells using α β tubulin antibody left and gst antibody 2622 right. Can be blocked with alpha tubulin peptide ab23537. Anti alpha tubulin antibody tu 02 is recommended for detection of α tubulin of mouse rat human and porcine origin by wb ip if ihc p fcm and elisa.
The gene codes for a member of the α tubulin family and contains 448 amino acids. Go to your regional site. Electrotransfer to nitrocellulose membrane. After electrophoresis the blot was stained with bodipy tr x succinimidyl ester red signal to detect total protein.
Samples of protein molecular weight standards product p 6649 containing decreasing amounts of a tubulin were run on an sds polyacrylamide gel and blotted onto a pvdf membrane. Electrotransfer to nitrocellulose membrane. Immunohistochemical analysis of paraffin embedded human breast carcinoma using α β tubulin antibody. After staining the blot was incubated with a mouse monoclonal anti a tubulin antibody.
α tubulin antibody tu 02 is a mouse monoclonal igm kappa light chain provided at 200 µg ml. The pa5 19489 immunogen is synthetic peptide conjugated to klh derived from within residues 400 to the c terminus of human alpha tubulin. Membrane blocking and. Detects a band of approximately 50 kda predicted molecular weight.
Go to your regional site. Polyclonal antibody for studying tubulin alpha in the cytoskeletal signaling research area. α tubulin also called tubulin α 4a tuba4a is mapped to human chromosome 2q35. Go to your regional site.
Pa5 19489 detects alpha tubulin which has a predicted molecular weight of approximately 50 kda. Electrotransfer to nitrocellulose membrane.