Alpha Tubulin Molecular Weight Kda

Use a concentration of 1 µg ml.

Alpha tubulin molecular weight kda. Most of these tubulins have distinct subcellular localization and an emerging diverse set of functions. Can be blocked with alpha tubulin peptide ab23537. α subunit of tubulin has molecular weight of 50 000. Tubulin is a heterodimer that consists of α tubulin and β tubulin.

Detects a band of approximately 50 kda predicted molecular weight. Membrane blocking and antibody incubations. To form microtubules the dimers of α and β tubulin bind to gtp and assemble onto the ends of microtubules while in the gtp bound state. Tubulin is the major constituent of microtubules.

Loading of prestained molecular weight markers 13953 5 µl lane to verify electrotransfer and biotinylated protein ladder 7727 10 µl lane to determine molecular weights are recommended. Tuba4a belongs to the subfamily of tubulin which is the major building block of microtubules. Pa5 19489 detects alpha tubulin which has a predicted molecular weight of approximately 50 kda. Detects a band of approximately 50 kda predicted molecular weight.

50 kda and share considerable homology. α tubulin also called tubulin α 4a tuba4a is mapped to human chromosome 2q35. The β tubulin subunit is exposed on the plus end of the microtubule while the α tubulin subunit is exposed on the minus end. Use at an assay dependent concentration.

The gene codes for a member of the α tubulin family and contains 448 amino acids. The α subunit of tubulin has molecular weight of 50 000. Electrotransfer to nitrocellulose membrane. Detects a band of approximately 55 kda predicted molecular weight.

Both subunits have a molecular weight of approx. We recommend using 3 bsa as the blocking agent blocking with milk may cause a reduction in signal intensity. Use a concentration of 1 µg ml. It binds two moles of gtp one at an exchangeable site on the beta chain and one at a non exchangeable site on the alpha chain.

Tubulin is the major constituent of microtubules. Works under both reducing and non reducing conditions. Prestained protein marker broad range 11 190 kda. In addition to α and β tubulin several other tubulins have been identified bringing the number of distinct tubulin classes to seven.

Each has a molecular weight of approximately 50 kda. Perform heat mediated antigen retrieval before commencing with ihc staining protocol. Volumes are for 10 cm x 10 cm.